The lab says fixation will induce the dyes to leak within the cells. I believe the belief is usually that fixation may perhaps destruction mobile membranes. I did locate a paper which describes fixation making use of methanol or acetic acid, following staining using a DCFDA spinoff. DCFDA could be https://myleswjifc.fitnell.com/70058135/not-known-factual-statements-about-2-fdck-hcl