Discard the supernatant and resuspend cell pellets with one hundred μL of intracellular staining antibody combine. Create an individual-stained cell Handle for the Fixable Viability Dye by warmth-killing 100 μL of only one-stained mobile Command sample as explained in steps 12c and d. Smash the spleen by using the plunger https://csgohealth.com/how-ozone-generators-can-improve-indoor-air-quality/