The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR solutions have been digested and cloned right into a pET21a+ vector employing NdeI and XhoI restriction enzymes. A point mutation was released by the fast https://voni777eqb2.newsbloger.com/profile